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Figure 4

From: Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells

Figure 4

Analysis of focal adhesion orientation and shape during wound healing. a Schematic representation of wound healing experiment. b Orientation of the wound in comparison to 1 μm topographical features and the pFAK immunolabelling (inverted grey scale) of migrating wound edge at different time points in orthogonal, parallel and flat configurations. Scale bars are 20 μm. c Graphical representation of orientation angle and aspect ratio parameters. d and e, Orientation angle and aspect ratio at different time points in orthogonal, parallel and flat configurations. The numbers of the analysed events per group are reported in the Methods section. *0,01

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