Figure 7 | Scientific Reports

Figure 7

From: Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells

Figure 7

Influence of cell-cell junctions in the topography-aided cell migration. a Phase contrast images of the advancing wound edge at different time points (0h, 12h, 24h, 36h) in WT MDCK cells + H3 antibody (control), WT MDCK cells + E-cadherin blocking antibody DECMA1, and ZO-1/ZO-2 KO MDCK cells + E-cadherin blocking antibody DECMA1 both on flat substrates, as well as on orthogonal microtopography. On the right side, standard deviation projection of all the collected images showing the advancing fronts during 0 h - 48 h of wound healing. b PIV analysis of the collective migration of the MDCK cells at 24 h time point on flat substrate and orthogonal topography.

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