Figure 3

Ultrastructural analysis of type1 and type2 NI. (a) TEM image of a type1 intranuclear inclusion (NI) in NAFLD shows a hepatocyte nucleus (N) with NI coated by a membrane resembling the double-layer nuclear membrane (arrows). The NI contains three (hetero-) lysosomes (triangle), a lipid droplet (LD) and an artificial hole (+) in the sample. (b) The image reveals a type2 NI with deposit of glycogen (*). The nuclear membrane (arrow) delimiting the nucleus is shown and a lipid droplet (LD) within the cytoplasm. (c,d) The images depict additional examples of a type1 NI (c) and a type2 NI (d). Ultrathin sections were mounted on copper grids, double-stained with uranyl acetate (1%) and lead citrate (0.4%). Electron microscopy scale bars: Image A: 1 µm and 0.5 µm (in increased magnification); Image B: 5 µm and 1 µm (in increased magnification); Image C: 2 µm; Image D: 5 µm.