Figure 5

Immunohistochemical analysis for autophagy-associated proteins in type1 and type2 NI. Images depict positive immunoreactivity (brown) for autophagy-associated proteins within type1 NI, whereby type2 NI are all immunonegative. Analysis of type1 NI demonstrates strong accumulation of p62, LC3B, ubiquitin, cathepsin B and cathepsin D within the inclusions. Regarding p62, LC3B and ubiquitin this immunopositivity is almost exclusively within the inclusions and nearly lacking in the cytoplasm. Examination of type2 NI shows that these inclusions do not contain any autophagy-associated proteins. The white bar at the bottom left indicates 20 µm and the white bars at the bottom middle and right equals 10 µm. Original magnifications: 400 X (left) and 1,000 X (right).