Figure 6
From: Characterization of two types of intranuclear hepatocellular inclusions in NAFLD
Three-dimensional imaging of double immunofluorescence-labelled (ubiquitin and p62) tissue sections in X-, Y- and Z-axis. A total of 152 optical sections of 0.1 µm were imaged and 98 of them were used for 3D reconstruction. The image demonstrates a representative case with type1 NI. DAPI staining (blue) depicts the nucleus with an intranuclear inclusion, which contains the autophagy-associated proteins ubiquitin and p62. Additionally, co-localizations of ubiquitin and p62 in the same inclusion are seen, proven by the formation of the strong merged colour yellow (merged image); ubiquitin = red, p62 = green.
