Table 1 Results from ten-temperature melting analysis of ClPPase variants.
From: IMPROvER: the Integral Membrane Protein Stability Selector
Module | Variant | \(T_m\) (\(^{\circ }\)C)\(^{\mathrm{a}}\) | \(T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{b}}\) | \(\Delta T_m\) (\(^{\circ }\)C) | \(\Delta T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{c}}\) | Repeats (n) | Status |
|---|---|---|---|---|---|---|---|
n/a | Wild-type | 49.3 | 0.9 | 0.0 | 1.2 | 8 | n/a |
Deep-sequence | S113A | 53.6 | 2.8 | 4.3 | 3.0 | 3 | Stabilising |
Deep-sequence | S273V | 48.8 | – | − 0.5 | – | 1 | Neutral |
Deep-sequence | G130S | 57.4 | – | 8.1 | – | 1 | Stabilising |
Deep-sequence | S371K | 50.6 | – | 1.3 | – | 1 | Neutral |
Deep-sequence | F20Y | 58.4 | 1.2 | 9.1 | 1.5 | 3 | Stabilising |
Model-based | V81W | 50.5 | – | 1.2 | – | 1 | Stabilising |
Model-based | R463W | 51.1 | 1.3 | 1.8 | 1.6 | 3 | Neutral |
Model-based | L142P | 44.0 | 0.5 | − 5.3 | 1.0 | 3 | Destabilising |
Model-based | L151I | 49.7 | 1.2 | 0.4 | 1.5 | 2 | Neutral |
Model-based | V693Y | 51.4 | – | 2.1 | – | 1 | Stabilising |
Model-based | R109W | 52.7 | 1.7 | 3.4 | 1.9 | 3 | Stabilising |
Model-based | R290F | 50.5 | – | 1.2 | – | 3 | Stabilising |
Model-based | D468F | 52.0 | 0.6 | 2.7 | 1.1 | 1 | Stabilising |
Model-based | I501L | 50.6 | – | 1.3 | – | 1 | Neutral |
Model-based | G179A | 52.9 | 1.5 | 3.6 | 1.7 | 3 | Stabilising |
Data-driven | A492L | 47.7 | – | − 1.6 | – | 1 | Destabilising |
Data-driven | A14L | 48.4 | – | − 0.9 | – | 1 | Neutral |
Data-driven | G31A | 51.0 | – | 1.7 | – | 1 | Stabilising |
Data-driven | A319L | 51.5 | – | 2.2 | – | 1 | Stabilising |
Data-driven | G130A | 62.3 | 1.1 | 13.0 | 1.4 | 3 | Stabilising |
Data-driven | A114L | 50.2 | – | 0.9 | – | 1 | Neutral |
Combined | G130A+F20Y | 59.6 | 1.8 | 10.3 | 2.0 | 3 | Stabilising |
