Table 2 Results from ten-temperature melting analysis of hENT1 variants.
From: IMPROvER: the Integral Membrane Protein Stability Selector
Module | Variant | \(T_m\) (\(^{\circ }\)C)\(^{\mathrm{a}}\) | \(T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{b}}\) | \(\Delta T_m\) (\(^{\circ }\)C) | \(\Delta T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{c}}\) | Repeats (n) | Status |
|---|---|---|---|---|---|---|---|
n/a | Wild-type | 44.6 | 0.7 | 0.0 | 0.9 | 12 | n/a |
Deep-sequence | I380V | 42.0 | 0.1 | − 2.5 | 0.6 | 2 | Destabilisng |
Deep-sequence | M306T | 41.3 | 0.1 | 0.4 | 1.8 | 2 | Neutral |
Deep-sequence | G225V | 42.1 | 0.1 | − 1.0 | 0.9 | 2 | Destabilisng |
Deep-sequence | S321T | 44.9 | 1.7 | − 2.5 | 0.6 | 2 | Destabilisng |
Model-based | R233L | 37.1 | 1.6 | − 3.8 | 0.6 | 2 | Destabilisng |
Model-based | S152L | 42.7 | 1.3 | − 1.9 | 1.4 | 5 | Destabilisng |
Model-based | E247M | 45.8 | 0.6 | 1.2 | 0.8 | 4 | Stabilising |
Model-based | N30F | 46.8 | 0.4 | 2.2 | 0.8 | 5 | Stabilising |
Model-based | L27E | 45.5 | 0.7 | 0.9 | 0.9 | 5 | Stabilising |
Data-driven | A401L | 46.3 | 0.7 | 1.7 | 1.0 | 2 | Stabilising |
Data-driven | T336A | 43.9 | 0.7 | − 0.7 | 0.9 | 5 | Neutral |
Data-driven | Q246A | 47.6 | 1.1 | 0.2 | 0.9 | 5 | Neutral |
Data-driven | F153A | 40.8 | 0.1 | − 7.5 | 1.7 | 2 | Destabilisng |
Data-driven | G207A | 43.6 | 0.7 | − 3.3 | 0.6 | 2 | Destabilisng |
Data-driven | A88L | 45.2 | 0.7 | 0.8 | 0.9 | 5 | Neutral |
Data-driven | K263A | 47.2 | 1.1 | 2.6 | 1.3 | 5 | Stabilising |
