Table 3 Results from ten-temperature melting analysis of \(\hbox {hPTH}_{1}\hbox {R}\) variants.
From: IMPROvER: the Integral Membrane Protein Stability Selector
Module | Variant | \(T_m\) (\(^{\circ }\)C)\(^{\mathrm{a}}\) | \(T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{b}}\) | \(\Delta T_m\) (\(^{\circ }\)C) | \(\Delta T_m\) error (\(\pm ^{\circ }\)C)\(^{\mathrm{c}}\) | Repeats (n) | Status |
|---|---|---|---|---|---|---|---|
n/a | Wild-type | 38.7 | 0.8 | 0 | 1.1 | 3 | n/a |
Deep-sequence | G188Y | 41.2 | 0.7 | 2.5 | 0.7 | 3 | Stabilising |
Deep-sequence | M189L | 39.1 | 0.9 | 0.4 | 0.9 | 3 | Neutral |
Deep-sequence | G188K | 40.3 | 1.2 | 1.6 | 1.2 | 3 | Stabilising |
Deep-sequence | A274D | 39.1 | 0.1 | 0.4 | 0.1 | 3 | Neutral |
Model-based | S198M | 42.1 | 0.8 | 3.4 | 1.1 | 3 | Stabilising |
Model-based | F291T | 40.9 | 0.9 | 2.2 | 1.2 | 3 | Stabilising |
Model-based | D251R | 41.7 | 1.5 | 3.0 | 1.6 | 3 | Stabilising |
Model-based | E260R | 39.7 | 1.5 | 1.0 | 1.7 | 3 | Neutral |
Data-driven | Q401A | 40.7 | 0.2 | 2.0 | 0.2 | 3 | Stabilising |
Data-driven | F288A | 41.5 | 0.7 | 2.8 | 0.7 | 3 | Stabilising |
Data-driven | E391A | 39.4 | 0.2 | 0.7 | 0.2 | 3 | Neutral |
Data-driven | G323A | 41.5 | 0.6 | 2.8 | 0.6 | 2 | Stabilising |
