Figure 5 | Scientific Reports

Figure 5

From: Simultaneous molecular MRI of extracellular matrix collagen and inflammatory activity to predict abdominal aortic aneurysm rupture

Figure 5

Spatial localization of the collagen 1-specific gadolinium-based probe and iron oxide particles in AAAs using Laser Ablation Inductively Coupled Mass Spectrometry (LA-ICP-MS). (A1) For an anatomical overview of the histological AAA section (A2), the phosphorus distribution was measured using LA-ICP-MS. (A3) LA-ICP-MS confirmed in vivo measurements with the visualization of gadolinium (from the collagen 1-specific gadolinium-based probe) in the aneurysmal wall. A co-localization of gadolinium accumulation with collagen fibers was also demonstrated using Picrosirius red staining (A4). (A5) LA-ICP-MS furthermore confirmed the accumulation of the iron in the aneurysmal wall. Additionally, the co-localization of iron accumulation was demonstrated with Perls’ Prussian blue staining (A6). AAA: abdominal aortic aneurysm. (B) In competition experiments, a significant decrease in CNR was found after administration of a collagen peptide (competition) without gadolinium labelling compared to the administration of the gadolinium-based collagen-targeted probe. CNR contrast-to-noise ratio.

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