Figure 5 | Scientific Reports

Figure 5

From: Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease

Figure 5

Sema3A restrains the size of synapses. (A) Gut explants cocultured with eGFP- or Sema3A-expressing COS-7 cells were immunolabeled with synapsin 1 and TuJ1 antibodies. Images at the bottom represent higher magnification of the boxed region. Scale bar 35 µm. (B,C) Quantification of the number of synapsin 1 clusters per 100 µm2 of Tuj1 labeling (B) and area of synapsin 1 clusters (C). Data are mean ± SEM (n = 5); *p = 0.032, Mann–Whitney test.

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