Figure 5 | Scientific Reports

Figure 5

From: Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture

Figure 5

Reference gene expression stability analysis in white adipose tissue (WAT) at three (A–D) and seven days (E–H) following surgery. (A/E) Distribution of threshold cycle (Ct) values for each group (Fx = fracture; TBI = traumatic brain injury; TBI + Fx = combined injury) of all candidate reference genes tested. (B/F) Pairwise variation (Vn/n+1) analysis by GeNorm, to determine the optimal number of reference genes for normalisation. (C/G) Stability analysis by NormFinder (stability value: sv), GeNorm (M value) and BestKeeper (coefficient of correlation: r, standard deviation: SD), with a sum displayed in the final rank (green = most stable, red = least stable). (D/H) Overall expression stability confirmation of RefFinder, which combines NormFinder, GeNorm, BestKeeper and the comparative delta-Ct method.

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