Table 2 qPCR primer set (murine) of all target genes tested.

From: Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture

Symbol

Gene name

Protein function

Primer sequence (F: 5′-3′/R: 3′-5′)

ApoE

Apolipoprotein E

Major apoprotein of the chylomicron, essential for the normal catabolism of lipoprotein constituents with triglycerides

F: TGGTGTTCAAAGACAATTTTTCCCT

R: CCACTCGAGCTGATCTGTCAC

Atf4

Activating transcription factor 4

Transcription factor, also characterised as the cAMP-response element binding protein 2 (Creb2)

F: CACTGGCGAGTGTAAGGAGC

R: TTCTTCCCCCTTGCCTTACG

Creb1

cAMP responsive element binding protein 1

Phosphorylation-dependent transcription factor

F: CCCTGCCATCACCACTGTAA

R: GGTTAATGTCTGCAGGCCCT

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