Figure 4 | Scientific Reports

Figure 4

From: In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging

Figure 4

Macrophage and dendritic cell ex vivo verification using TPE-FLIM and bright field microscopy. Side by side comparison of TPE-FLIM images (mean fluorescence lifetime τm in the range 300–1,300 ps) (a, c, e) and corresponding light microscopic images (b, d, f) after staining of human skin biopsy cryo-sections for M1 macrophages with CD68 (b), M2 macrophages with CD163 (d) and dendritic cells with CD11c (f) and counterstained nuclei with Mayer's hemalum solution. MCs are marked with orange circles, macrophages and dendritic cells with white/gray circles, respectively. The laser-burned labels are 28 µm × 28 µm. Images have been rotated and zoomed to match the same orientation and size. Scale bar: 30 µm.

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