Table 1 TPE-FLIM parameters τ1, τ2, τm, a1/a2 and TPE-AF intensity of the pure and IgE-sensitized resting and activated/degranulated MCs, of the main dermal cells measured in vitro in the cell culture and of the shorter and longer τm MCs measured in vivo on the volar forearm of healthy volunteers, allergic and mastocytosis patients and ex vivo in human skin cryo-sections.
Number of cells | τm in ps | τ1 in ps | τ2 in ps | a1/a2 | TPE-AF intensity, photons/mW | |
|---|---|---|---|---|---|---|
Resting MCs | 43 | 1,248 ± 287 | 533 ± 266 | 2,289 ± 317 | 1.5 ± 0.5 | 1,300 ± 400 |
Activated/degranulated MCs | 13 | 862 ± 268 | 288 ± 130 | 1920 ± 287 | 2.5 ± 2.0 | 900 ± 200 |
IgE-sensitized resting MCs | 14 | 1,107 ± 54 | 412 ± 83 | 2,427 ± 256 | 1,9 ± 0.3 | 800 ± 80 |
IgE-sensitized activated/degranulated MCs | 15 | 747 ± 44 | 217 ± 21 | 1877 ± 117 | 2.1 ± 0.6 | 1,000 ± 120 |
Macrophages (short lifetime) | 34 | 461 ± 175 | 225 ± 84 | 1,289 ± 278 | 4.8 ± 3.4 | 3,000 ± 500 |
Macrophages (long lifetime) | 20 | 1,348 ± 188 | 1,016 ± 220 | 2,432 ± 239 | 2.6 ± 1.0 | 800 ± 200 |
Dendritic cells | 14 | 1,265 ± 180 | 434 ± 188 | 2,578 ± 328 | 1.6 ± 0.2 | 538 ± 258 |
Fibroblasts | 6 | 921 ± 81 | 429 ± 51 | 1983 ± 137 | 0.5 ± 0.1 | 469 ± 137 |
Neutrophils | 21 | 1,074 ± 109 | 714 ± 250 | 1795 ± 600 | 1.5 ± 0.5 | 500 ± 115 |
In vivo (healthy skin) MCs (longer τm) | 71 | 1,171 ± 117 | 344 ± 49 | 2,301 ± 77 | 1.5 ± 0.3 | 300 ± 40 |
In vivo (healthy skin) MCs (shorter τm) | 48 | 678 ± 151 | 241 ± 59 | 1947 ± 230 | 3.0 ± 1.1 | 800 ± 100 |
In vivo (mastocytosis skin) MCs (longer τm) | 9 | 1,211 ± 130 | 360 ± 76 | 2,243 ± 141 | 1.3 ± 0.2 | 280 ± 40 |
In vivo (mastocytosis skin) MCs (shorter τm) | 3 | 597 ± 136 | 173 ± 31 | 1902 ± 206 | 3.5 ± 0.8 | 700 ± 100 |
In vivo (allergy skin) MCs (longer τm) | 8 | 970 ± 157 | 310 ± 58 | 2,204 ± 103 | 1.8 ± 0.7 | 320 ± 120 |
In vivo (allergy skin) MCs (shorter τm) | 9 | 546 ± 89 | 185 ± 20 | 1925 ± 147 | 4.6 ± 1.1 | 840 ± 160 |
Ex vivo (skin biopsy) MCs (longer τm) | 9 | 1,204 ± 97 | 417 ± 86 | 2,181 ± 166 | 1.7 ± 1.0 | 1,600 ± 300 |
Ex vivo (skin biopsy) MCs (shorter τm) | 4 | 730 ± 140 | 243 ± 43 | 1743 ± 167 | 2.4 ± 0.7 | 2000 ± 300 |