Table 1 TPE-FLIM parameters τ1, τ2, τm, a1/a2 and TPE-AF intensity of the pure and IgE-sensitized resting and activated/degranulated MCs, of the main dermal cells measured in vitro in the cell culture and of the shorter and longer τm MCs measured in vivo on the volar forearm of healthy volunteers, allergic and mastocytosis patients and ex vivo in human skin cryo-sections.

From: In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging

 

Number of cells

τm in ps

τ1 in ps

τ2 in ps

a1/a2

TPE-AF intensity, photons/mW

Resting MCs

43

1,248 ± 287

533 ± 266

2,289 ± 317

1.5 ± 0.5

1,300 ± 400

Activated/degranulated MCs

13

862 ± 268

288 ± 130

1920 ± 287

2.5 ± 2.0

900 ± 200

IgE-sensitized resting MCs

14

1,107 ± 54

412 ± 83

2,427 ± 256

1,9 ± 0.3

800 ± 80

IgE-sensitized activated/degranulated MCs

15

747 ± 44

217 ± 21

1877 ± 117

2.1 ± 0.6

1,000 ± 120

Macrophages (short lifetime)

34

461 ± 175

225 ± 84

1,289 ± 278

4.8 ± 3.4

3,000 ± 500

Macrophages (long lifetime)

20

1,348 ± 188

1,016 ± 220

2,432 ± 239

2.6 ± 1.0

800 ± 200

Dendritic cells

14

1,265 ± 180

434 ± 188

2,578 ± 328

1.6 ± 0.2

538 ± 258

Fibroblasts

6

921 ± 81

429 ± 51

1983 ± 137

0.5 ± 0.1

469 ± 137

Neutrophils

21

1,074 ± 109

714 ± 250

1795 ± 600

1.5 ± 0.5

500 ± 115

In vivo (healthy skin) MCs (longer τm)

71

1,171 ± 117

344 ± 49

2,301 ± 77

1.5 ± 0.3

300 ± 40

In vivo (healthy skin) MCs (shorter τm)

48

678 ± 151

241 ± 59

1947 ± 230

3.0 ± 1.1

800 ± 100

In vivo (mastocytosis skin) MCs (longer τm)

9

1,211 ± 130

360 ± 76

2,243 ± 141

1.3 ± 0.2

280 ± 40

In vivo (mastocytosis skin) MCs (shorter τm)

3

597 ± 136

173 ± 31

1902 ± 206

3.5 ± 0.8

700 ± 100

In vivo (allergy skin) MCs (longer τm)

8

970 ± 157

310 ± 58

2,204 ± 103

1.8 ± 0.7

320 ± 120

In vivo (allergy skin) MCs (shorter τm)

9

546 ± 89

185 ± 20

1925 ± 147

4.6 ± 1.1

840 ± 160

Ex vivo (skin biopsy) MCs (longer τm)

9

1,204 ± 97

417 ± 86

2,181 ± 166

1.7 ± 1.0

1,600 ± 300

Ex vivo (skin biopsy) MCs (shorter τm)

4

730 ± 140

243 ± 43

1743 ± 167

2.4 ± 0.7

2000 ± 300