Figure 2

Histological sections of normal small veins, side branches of the saphenous vein at contralateral side (a) and morphologically dilated veins found in reticular vein plaques (b). Stained with (left to right) smooth muscle actin immunochemistry (brown, DAB), resorcin fuchsin (magenta, elastica) and PicroSirius (collagen, red). Scale bar, 100 µm. (c) Quantitative evaluation of wall tissue components in normal side branches and in reticular veins. Density histograms for blue (suppressed by SMA DAB brown) and green (suppressed by RF’s magenta elastica and PS’s red collagen) colors. Relative frequency of staining intensity is given in thousands of venous wall cross sections.