Figure 3
Gene structure and protein motif analysis of BdSBP genes. (A) Un-rooted neighbour-joining tree was developed using SBP domain sequences through MEGA6 package. The organizations of exon–intron and intron phases of the BdSBP genes are displayed. Exons, introns and 5′UTR/3′UTR are denoted by red boxes, horizontal black lines and black boxes, respectively. *miR156 targeted BdSBP genes. (B) The conserved motifs in BdSBP proteins are shown in different colors. Full length protein sequences of BdSBPs were used to search motif using MEME tool. (C) Functions of BdSBP genes are annotated based on Gene ontology. The biological processes (BP), molecular functions (MF), and cellular component (CC) are shown in the box below. Biological Processes (BP): 1. GO:0006355; regulation of transcription, DNA-templated; 2. GO:0010229;inflorescence development; 3. GO:0010228;vegetative to reproductive phase transition of meristem; 4. GO:0010321;regulation of vegetative phase change; 5. GO:0009911;positive regulation of flower development; 6. GO:0055070;copper ion homeostasis; 7. GO:0048638;regulation of developmental growth; 8. GO:0035874;cellular response to copper ion starvation; 9. GO:0048510;regulation of timing of transition from vegetative to reproductive phase; 10. GO:0048653;anther development; 11. GO:0045893;positive regulation of transcription, DNA-templated 12. GO:0010358;leaf shaping 13. GO:0009556;microsporogenesis; 14. GO:0009554;megasporogenesis; 15. GO:0042127;regulation of cell proliferation; 16. GO:2000025;regulation of leaf formation; 17. GO:0008361;regulation of cell size;18. GO:0042742;defense response to bacterium; 19. Unknown (U). Molecular function (MF): 1.GO:0003677; DNA binding; 2. GO:0005515;protein binding; 3. GO:0003700; transcription factor activity, sequence-specific DNA binding; 3. GO:0043565; sequence-specific DNA binding; 4. GO:0042803;protein homodimerization activity; 5. GO:0044212; transcription regulatory region DNA binding; 6. unknown (U). Cellular Component (CC): 1. GO:0005634;Nucleus; 2. GO:0009941;chloroplast envelope 3. GO:0016607;nuclear speck; 4. GO:0005886;plasma membrane 5. Unknown (U).
