Figure 5
Analysis of post-transcriptional regulation of BdSBP genes by miR156 and AS. (A) Prediction of miR156 target site in BdSBPs transcripts. The SBP domain is represented in green. miR156 complementary sequences of BdSBPs are indicated in red. 5′ and 3′-UTRs are indicated in black horizontal lines. (B) Details of AS events in miR156 targeted and non-targeted BdSBP genes. X-axis shows BdSBP genes and Y-axis indicates number of transcripts. (C) Agarose gel image showing product of 5′-RACE PCR to measure transcript cleavage of BdSBP genes by miR156. (D) miR156 mediated cleavage site mapping in BdSBP3, BdSBP17 and BdSBP23 genes. The arrow indicates exact cleavage site and number indicates clones used for confirmation. (E) Semi-quantitative RT-PCR analysis of BdSBP genes in leaf and spike tissues. BdUBC18 was used as an internal control. miR156 targeted BdSBP genes expressed poorly in leaf and higher in spike.
