Figure 2 | Scientific Reports

Figure 2

From: Forensic tracers of exposure to produced water in freshwater mussels: a preliminary assessment of Ba, Sr, and cyclic hydrocarbons

Figure 2

The principle component analysis score plot of aligned GC × GC-TOFMS peak lists from produced water (pink circles) and fatty tissue of mussels exposed to both high (green circles) and low doses (blue circles) of the same produced water from hydraulic fracturing operations. Fatty tissue from mussels that were not exposed are shown in red circles, and method blanks are in light blue circles. Note that the exposed fatty tissue samples score separately than both the unexposed control tissue samples and method blanks. Over 70% of the score difference between the control and the exposed mussels is attributable to cyclic hydrocarbons. Analytes detected by GC × GC-TOFMS were subjected to Kruskal–Wallis ANOVA and p < 0.05 was required for PCA modeling; shading represents the 95% confidence interval.

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