Table 1 Inhibition of HU protein DNA-binding by synthesized BDFs.

HU protein

IC₅₀, μM (average ± SD from three runs of EMSA)

HUSpm

5.6 ± 0.8

6.3 ± 0.7

8.2 ± 1.1

HUMgal

5.0 ± 0.6

5.9 ± 0.8

8.1 ± 1.0

HUEc

7.5 ± 1.0

7.5 ± 0.9

10 ± 1.3

Activities of BDF4 against HUSpm mutants, IC₅₀, μM (mean ± SD from three runs of EMSA)

HUSpm_R57A

HUSpm_K80A

HUSpm_R88A

HUSpm_K35T

6.3 ± 1.0

5.9 ± 0.9

6.3 ± 1.2

1.8 ± 0.3