Table 2 Dissociation constants (Kd in nM, average ± SD, n = 2) for HUSpm wild type and HUSpm bearing mutations R57A, K80A and K88A binding to various DNA structures calculated from EMSA experiments (see Fig. 5B legend for a description of the DNA sequences).

From: Structure-based inhibitors targeting the alpha-helical domain of the Spiroplasma melliferum histone-like HU protein

DNA

HUSpm wild type

HUSpm_R57A

HUSpm_K80A

HUSpm_K88A

Nick (N)

5.2 ± 1.1

398 ± 45

69 ± 20

220 ± 40

DS48 (48)

18.6 ± 4.8

788 ± 100

163 ± 27

390 ± 55

A1 bulge (A1)

2.5 ± 0.6

175 ± 25

41 ± 8

83 ± 15

A7 bulge (A7)

2.0 ± 0.5

121 ± 15

13 ± 3

51 ± 8

DS24a (24a)

2.6 ± 0.6

230 ± 30

40 ± 10

84 ± 20

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