Figure 2 | Scientific Reports

Figure 2

From: Extracellular vesicles produced by immunomodulatory cells harboring OX40 ligand and 4-1BB ligand enhance antitumor immunity

Figure 2

Characterization of EVs isolated from genetically modified B16F10 tumor cells by flow cytometry. The EVs preparations, adsorbed on the beads surface, were labeled with anti-CD63, anti-CD9 and anti-CD81 antibodies. The CD9 and CD81 proteins show greater protein expression in relation to CD63. The difference in the expression of these proteins was evidenced by the shift of the (green) peak to the right, compared to their controls (peak in red), labeled with isotype antibodies, IgG2ĸ. (A) In these representative histograms, the y-axis represents the percentage of EV numbers and the x-axis is the mean fluorescence intensity (MFI) on a logarithmic scale. (B) Graphic representation of the percentage of positive EVs for each indicated labeling. The bars represent the mean ± SD of three independent experiments. There was no statistical difference of tetraspanins labeling between different groups. Two-way ANOVA test was performed.

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