Figure 3

Graphical representation of CD3-positive lymphocytes in the lung parenchyma (cells 10^–4/µm²) and spleen red and white pulp (µm²/µm²). Each point represents one animal and bars show median. Representative photomicrographs of immunostained CD3 lymphocytes in the lung parenchyma (20 ×, scale bars 50 μm) and spleen (10 ×, scale bars 100 μm): the CD3-positive lymphocyte content of the LPS5w group was increased compared to that of the control, PM and LPS + PM groups. The same pattern was observed for circulating and BALF lymphocytes. *p < 0.05 compared to the LPS5w group. The two-way ANOVA analyses showed that the CD3-positive lymphocyte count in the lung parenchyma was influenced by the LPS nebulization and PM exposure. The spleen CD3-positive lymphocyte counts in the white and red pulp were influenced by the LPS nebulization, PM exposure and their interaction.