Figure 2

(A) Thin layer chromatography of the lipids in the supernatant of the lipid exchange process. The concentration of MαCD was 20 mM. The loaded lipids were present in excess in the supernatant of the exchange process, causing large dark bands on the TLC plate. In lanes 1 and 2, the loaded lipid was bSM at 0.375 mM and 0.75 mM, respectively. In lanes 3 and 4 the loaded lipid was POPC at 0.375 mM and 0.75 mM, respectively. In lanes 5 and 6 the loaded lipid was POPS at 0.75 mM and 1.5 mM, respectively. The concentration of loaded lipids in the odd numbered lanes is twice the concentration of loaded lipids in the even numbered lanes. Lane 7 shows bands for total phospholipid lipid extraction of RBCs. (B) Thin layer chromatography of total lipid extracts of RBCs after the exchange. In the first three lanes, RBCs from three different donors were exchanged with POPS (at a concentration of 0.75 mM). Lanes 4–6 are total lipid extracts of RBCs from the same donors as in lanes 1–3, respectively. Note that a different mobile phase was used for each plate, and therefore bands appear in a different order. The uncropped version of both TLC plates are presented in Supplementary Fig. S2.