Figure 5
Evaluation of the maintenance of antiparasitic effects of oleoresins by reversibility assay. T. gondii-infected BeWo cells were exposed to 64 and 32 µg/mL of oleoresins from C. reticulata, C. duckei, C. paupera, C. pubiflora, SDZ + PYR (200 + 8 μg/mL) and culture medium alone (control group/untreated group) for 24 h, followed by treatment removal for an additional 24 h. Parasite proliferation at 24 h of treatment with oleoresins was considered the baseline for comparison. (A) The reversibility ratio measures the ability of the parasites to recover from treatment and regain infectiveness. The ratio is shown in percentage change (% reversibility of treatment) in comparison with the control group, which was considered as 100% reversibility. The β-galactosidase assay was used as an indicator of parasite activity. (B) Parasites obtained from oleoresin-treated BeWo cells were harvested and used to infect new host cells for 24 h. The number of tachyzoites was determined by β-galactosidase assay and expressed as % of T. gondii proliferation. Data are shown as means ± standard deviation from experiments performed in eight replicates. *Comparison between infected/untreated cells and infected/treated cells; &Comparison to SDZ + PYR-infected/treated cells; $Comparison between either treatment concentrations of each species of Copaifera oleoresin. #Comparison with the respective treatment in the condition of 24 h of treatment. Significant differences were analyzed using one-way ANOVA and Tukey’s multiple comparisons post-test. Differences were considered significant when P < 0.05.
