Figure 8
Cytokine and ROS production by T. gondii-infected BeWo cells after treatments. BeWo cells were infected or not with T. gondii tachyzoites, followed by treatment for 24 h with 64 and 32 µg/mL of oleoresins from C. reticulata, C. duckei, C. paupera, C. pubiflora, SDZ + PYR (200 + 8 μg/mL) and culture medium only (control group/untreated group). Supernatants were collected for measurement of (A,B) IL-6 and (C,D) MIF. (E,F) BeWo cells were incubated with the probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA) and the ROS production was measured by flow cytometry and expressed as median fluorescence intensity (MFI). Data are expressed as means ± standard deviation from experiments performed in five replicates. *Comparison between uninfected/untreated cells (control) and uninfected/treated cells (A,C,E); *Comparison between infected/untreated cells (control T.g.) and infected/treated cells (B,D,F); #Comparison between uninfected/untreated cells (control) and infected/untreated cells (control T.g.); &Comparison in relation to the SDZ + PYR treatment. Significant differences were determined by using one-way ANOVA and Tukey’s multiple comparisons post-test. Differences were considered significant when P < 0.05.
