Figure 6 | Scientific Reports

Figure 6

From: Photoperiodic effects on monoamine signaling and gene expression throughout development in the serotonin and dopamine systems

Figure 6

RNAScope revealed significant differences in adult gene expression of relevant 5-HT genes in the DRN due to developmental photoperiod. Representative image for an animal that developed under a Long photoperiod and measured at P50: (A) Tph2 expression is stained in green and tagged with Alexa488, (B) SERT expression is stained in red and tagged with Atto550, (C) Pet-1 expression is stained in blue and tagged with Atto647, and (D) is the overlay of all three channels. Representative image for an animal that developed under a Short photoperiod and measured at P50: (E) Tph2 expression is stained in green and tagged with Alexa488, (F) SERT expression is stained in red and tagged with Atto550, (G) Pet-1 expression is stained in blue and tagged with Atto647, and (H) is the overlay of all three channels. Note that qualitatively, fluorescent levels are brighter under Short compared to Long photoperiod conditions. Scale bar was set at 100 μm.

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