Figure 3 | Scientific Reports

Figure 3

From: Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

Figure 3

Pathological LV hypertrophy and perivascular fibrosis post-SAC. Pathological hypertrophy marker mRNAs were evaluated one week post-sham or -SAC surgery in adult male C57BL/6J mice (9-week-old) from (a) LV tissue (sham, n = 7; SAC, n = 6) or (b) enriched cardiomyocyte fractions (sham, n = 7; SAC, n = 8). (c) Representative transverse LV sections demonstrate the development of perivascular fibrosis around the coronaries (indicated by black arrows) after SAC-surgery (right) compared to sham-surgery (left). Sections were stained with Fast Green and Picrosirius Red to identify the cytoplasm and collagen, respectively. (d) Hif1a and Vegfa expression in cardiomyocytes one week post-SAC (sham, n = 7; SAC, n = 8). Data are presented as means ± SD, independent comparisons were made by two-tailed Student’s unpaired t-tests; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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