Figure 2
From: Pancreatic β-cells respond to fuel pressure with an early metabolic switch
13C NMR spectrum of hyperpolarized metabolites after 4 h of incubation with 11.7 mM [U-13C,D] glucose. The metabolites were polarized for 90 min. after which they were subjected to rapid dissolution in hot phosphate buffer. Four metabolites were observed with distinct carbonyl shifts at: 2-13C-pyruvate (205.8 ppm), 1-13C-lactate (183.4 ppm), 5-13C-glutamate (182.2 ppm), 1-13C-alanine (176.8 ppm), 1-13C-glutamate (175.6 ppm) and 1-13C-pyruvate (171.2 ppm). The full spectrum is displayed in the insert where a grey box indicates the expanded region with the metabolites mentioned above. Other components in the sample were glucose, glycerol to mediate the hyperpolarization and an internal standard for quantification.
