Figure 1
From: Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility
Partial genomic DNA sequences of the six CmDMC1 genes of Chrysanthemum morifolium cultivar ‘Shuho-no-chikara’. Genomic DNAs corresponding to nucleotide positions 234–815 in the six cDNAs (Supplementary Fig. S1) are aligned with the DNASIS version 3.7 (Hitachi Software Engineering). Uppercase letters in blue indicate exon, and lower-case letters indicate intron. Asterisks (*) indicate the same nucleotides as in CmDMC1a; red letters indicate different nucleotides compared to CmDMC1a; red bar (-) indicates nucleotide deletion. TAL-L and TAL-R indicate the TALEN pair targeting CmDMC1 genes. A primer pair, DMC1-RNAi F1 and DMC1-RNAi R1, was used to amplify partial DNA fragments of individual CmDMC1 genes that correspond to cDNA nucleotide positions 234–815 of ‘Shuho-no-chikara’ (Supplementary Fig. S1). A primer pair, DMC1-gF1 and DMC1-gR1, was designed for the detection of mutations around the recognition sequences of the TALENs.
