Figure 3
From: Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility
Expression levels of CmDMC1 genes in CmDMC1-TALEN chrysanthemum plants. To detect endogenous CmDMC1 transcripts, total RNA was isolated from anthers and ovaries at early meiotic division stage before tetrad formation of CmDMC1-TALEN and non-transgenic control plants grown at 20 °C. Twenty μg of total RNA was applied to each lane. The RNA blots were probed with a 1032-bp fragment of the CmDMC1a cDNA as in Supplementary Fig. S1, and 1134-bp of the ACTIN cDNA of Ch. morifolium. SH and YS: RNA from non-transgenic controls ‘Shuho-no-chikara’ and ‘Yamate-shiro’, respectively. SH#12, SHa#13 and SHc#15 in panels A, C, E and G: TALEN lines from ‘Shuho-no-chikara’, and YS#16, YSa#12 and YSc#27 in panels B, F, D and H: those from ‘Yamate-shiro’. Panels A, B, C and D show RNA blots of anthers and panels E, F, G and H show those of ovaries. Their full-length blots are presented in Supplementary Fig. 5.
