Table 1 Mutation patterns of CmDMC1 alleles in leaves and roots.

From: Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility

Line^a	No. of mutated CmDMC1 alleles	Organ	Mutations detected in each CmDMC1 gene^b,c
Line^a	No. of mutated CmDMC1 alleles	Organ	CmDMC1a		CmDMC1b		CmDMC1c		CmDMC1d		CmDMC1e		CmDMC1f
SH#12	12	Leaf	m	25-d1	m	19-d1	m	25-d2	m	26-d1	m	22-d1	m	20-d1
		Leaf	m	25-d1	m	19-d1	m	40-i1	m	40-i1	m	22-d1	m	20-d1
		Root	m	25-d1	m	19-d1	m	25-d2	m	26-d1	m	22-d1	m	20-d1
		Root	m	25-d1	m	19-d1	m	40-i1	m	40-i1	m	22-d1	m	20-d1
SH#a13	10	Leaf	n	–	m	27-d2	m	25-d2	m	26-d3	m	23-d2	m	20-d1
		Leaf	n	–	m	27-d2	m	25-s1, 26-d2	m	28-d1	m	23-d2	m	40-i1
		Root	n	–	m	27-d2	m	25-d2	m	26-d3	m	23-d2	m	20-d1
		Root	n	–	m	27-d2	m	25-s1, 26-d2	m	28-d1	m	23-d2	m	40-i1
SH#b14	10	Leaf	m	25-d2	n	–	m	25-d1	m	26-d1	m	22-d2	m	20-d2
		Leaf	m	25-d2	n	–	m	25-d2	m	26-d1	m	26-d3	m	19-d2
		Root	m	25-d2	n	–	m	25-d1	m	26-d1	m	22-d2	m	20-d2
		Root	m	25-d2	n	–	m	25-d2	m	26-d1	m	26-d3	m	19-d2
SH#c15	10	Leaf	m	25-d1	m	19-d2	n	–	m	26-d2	m	22-d1	m	20-d2
		Leaf	m	40-i1	m	20-d2	n	–	m	40-i1	m	21-d2	m	20-d2
		Root	m	25-d1	m	19-d2	n	–	m	26-d2	m	22-d1	m	20-d2
		Root	m	40-i1	m	20-d2	n	–	m	40-i1	m	21-d2	m	20-d2
SH#d16	10	Leaf	m	24-d1	m	26-d2	m	26-d2	n	–	m	22-d1	m	22-d2
		Leaf	m	24-d1	m	26-d2, 28-s1	m	26-d2	n	–	m	22-d2	m	23-d2
		Root	m	24-d1	m	26-d2	m	26-d2	n	–	m	22-d1	m	22-d2
		Root	m	24-d1	m	26-d2, 28-s1	m	26-d2	n	–	m	22-d2	m	23-d2
SH#e19	10	Leaf	m	27-d2	m	26-d2, 28-s1	m	25-d1	m	24-d1	n	–	m	22-d1
		Leaf	m	27-d2	m	26-d2, 28-s1	m	24-d2	m	24-d1	n	–	m	22-d1
		Root	m	27-d2	m	26-d2, 28-s1	m	25-d1	m	24-d1	n	–	m	22-d1
		Root	m	27-d2	m	26-d2, 28-s1	m	24-d2	m	24-d1	n	–	m	22-d1
SH#f20	10	Leaf	m	25-d1	m	19-d1	m	26-d1	m	26-d2	m	23-d1	n	–
		Leaf	m	25-d1	m	19-d1	m	40-i1	m	26-d3	m	23-d1	n	–
		Root	m	25-d1	m	19-d1	m	26-d1	m	26-d2	m	23-d1	n	–
		Root	m	25-d1	m	19-d1	m	40-i1	m	26-d3	m	23-d1	n	–
YS#16	12	Leaf	m	24-d1	m	19-d2	m	22-d2	m	25-d2	m	22-d1	m	22-d2
		Leaf	m	24-d1	m	19-d2	m	22-d2	m	25-d2	m	22-d2	m	22-d2
		Root	m	24-d1	m	19-d2	m	22-d2	m	25-d2	m	22-d1	m	22-d2
		Root	m	24-d1	m	19-d2	m	22-d2	m	25-d2	m	22-d2	m	22-d2
YS#a12	10	Leaf	n	–	m	16-d2, 18-s1	m	22-d2	m	25-d2, 27-s1	m	22-d1	m	23-d1
		Leaf	n	–	m	16-d2, 18-s1	m	22-d2	m	24-d4	m	22-d1	m	23-d2
		Root	n	–	m	16-d2, 18-s1	m	22-d2	m	25-d2, 27-s1	m	22-d1	m	23-d1
		Root	n	–	m	16-d2, 18-s1	m	22-d2	m	24-d4	m	22-d1	m	23-d2
YS#b13	10	Leaf	m	27-d1	n	–	m	22-d1	m	23-d1	m	21-d1	m	22-d2
		Leaf	m	27-d2	n	–	m	22-d1	m	23-d1	m	21-d2	m	22-d2
		Root	m	27-d1	n	–	m	22-d1	m	23-d1	m	21-d1	m	22-d2
		Root	m	27-d2	n	–	m	22-d1	m	23-d1	m	21-d2	m	22-d2
YS#c27	10	Leaf	m	23-d1	m	22-d1	n	–	m	23-d1	m	22-d2	m	23-d1
		Leaf	m	23-d1	m	22-d2	n	–	m	40-i1	m	26-d3	m	22-d2
		Root	m	23-d1	m	22-d1	n	–	m	23-d1	m	22-d2	m	23-d1
		Root	m	23-d1	m	22-d2	n	–	m	40-i1	m	26-d3	m	22-d2
YS#d28	10	Leaf	m	22-d1	m	23-d2	m	22-d2	n	–	m	24-d1	m	22-d1
		Leaf	m	22-d2	m	22-d2, 24-s1	m	22-d2	n	–	m	24-d2	m	22-d1
		Root	m	22-d1	m	23-d2	m	22-d2	n	–	m	24-d1	m	22-d1
		Root	m	22-d2	m	22-d2, 24-s1	m	22-d2	n	–	m	24-d2	m	22-d1
YS#e30	10	Leaf	m	27-d1	m	19-d2	m	22-d1	m	23-d2	n	–	m	24-d1
		Leaf	m	40-i1	m	19-d2	m	22-d2	m	23-d2	n	–	m	24-d1
		Root	m	27-d1	m	19-d2	m	22-d1	m	23-d2	n	–	m	24-d1
		Root	m	40-i1	m	19-d2	m	22-d2	m	23-d2	n	–	m	24-d1
YS#f32	10	Leaf	m	22-d1	m	19-d1	m	23-d1	m	22-d2	m	24-d1	n	–
		Leaf	m	22-d1	m	19-d2	m	23-d2	m	22-d4	m	24-d1	n	–
		Root	m	22-d1	m	19-d1	m	23-d1	m	22-d2	m	24-d1	n	–
		Root	m	22-d1	m	19-d2	m	23-d2	m	22-d4	m	24-d1	n	–

^aSH: CmDMC1-edited lines from the cultivar ’Shuho-no-chikara’; YS: CmDMC1-edited lines from ‘Yamate-shiro’.
^bThe letters 'm' and 'n' indicate mutation and non-mutation, respectively, in the spacer region between the two TALEN binding sites.
^cA number indicates the nucleotide position of TALEN-binding sites.
d# indicates the number of nucleotides deleted, i1 and s1 indicate a nucleotide insertion and substitution, respectively.

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Table 1 Mutation patterns of CmDMC1 alleles in leaves and roots.

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