Figure 3
Phage infection assays. Phage CBA120 infection of E. coli strain ATCC 700,728 was followed by spectrophotometry at 600 nm as detailed in the methods section. The mean values of technical triplicate and the standard deviations are shown (a) Phage infection in the presence of various TSPs. The ability of CBA120 to infect E. coli O157:H7 treated with TSP1 (yellow circles), TSP2 (green circles), or TSP3 (blue circles) at 100 μg/mL is shown together with controls consisting of bacterial cells alone (black circles) and non-TSP treated bacterial cells incubated with phage (magenta circles). Only treatment with TSP2 prevented phage infection and subsequent bacterial death. (b) Phage CBA120 infection dependence on TSP2 concentration. Controls are the same as in (a). TSP2 at 25 and 100 μg/mL prevented killing by the phage but 5 μg/mL inhibited phage killing only for ~ 4.5 h.
