Figure 6

Prediction of putative kinases phosphorylating sites affected by Ppz1 overexpression. (a) The set of phosphosites hyperphosphorylated or dephosphorylated at any time upon overexpression of Ppz1 was submitted to the NetworKIN 3.0 high-throughput interface using the yeast Ensemble 74 database (min score = 2). If more than one kinase was assigned to a given site, only the kinase with the highest score was selected. Due to the similarity of Tpk1-3 consensus sites, the sum of all of them is also plotted. For phosphorylated sites, all kinases are shown. For dephosphorylated sites, only kinases with ≥ 2 sites are represented (the full table is available as Supplementary Table S6). (b) Motif enrichment using the MoMo software of phosphorylated sites, as described in Material and Methods. (*) according to the authors, the p-value of the Fisher Exact test on the enrichment of the motif should be interpreted as a score only. “Fold-Enr”, fold enrichment of the foreground matches vs. the background matches. (c) Time-dependent variation of motif enrichment for the dephosphorylated sites. In this case, only sites newly appearing at a given time were submitted to MoMo analysis, to better follow the variation in the motif pattern along the time of Ppz1 overexpression.