Figure 2
Perfluorooctanoic acid (PFOA) induced the secretion of proinflammatory cytokines by corneal epithelial cells, which affected inflammatory responses of corneal endothelial cells. (a) Inflammatory cytokines in the corneal epithelial cells (HCEpiC) basolateral media may alter the tight junction integrity of corneal endothelial cells (HCEC). Relative transepithelial electrical resistance of HCEC incubated with PBS (control), interleukin (IL) 6 (20 ng/mL), or HCEpiC basolateral media for 24 h. Experimental groups were compared using ANOVA (P = 0.0014), and Dunnett’s multiple comparisons tests were used for paired comparisons between control and IL-6- or HCEpiC basolateral media-treated HCEC. P values below 0.05 were considered to indicate statistical significance. (b) Inflammatory cytokines in the corneal epithelial cells (HCEpiC) basolateral media may induce the secretion of inflammatory cytokines by HCEC. Basolateral media of HCEpiC cells treated with PBS (control) or PFOA for 16 h were collected and used to treat HCEC for 16 h. The levels of IL-6 and IL-8 were determined using enzyme-linked immunosorbent assays. Unpaired t-test was used to determine significant differences between control and PFOA treated groups. P values below 0.05 were considered to indicate statistical significance. Asterisks denote statistical significance.
