Figure 2
From: Biological sex does not predict glymphatic influx in healthy young, middle aged or old mice
Male/female differences in influx rhythms. (a) Mean average intensity per animal over time under ketamine/xylazine anesthesia. Individual animals are colored dots, solid lines indicate significant cosinor fit. Red: female, blue: male. ZT: zeitgeber time, where ZT0 is lights on, ZT12 lights off. n = 29 female and 42 male mice, 4–9 mice per time point. (b) Comparison of 95% confidence intervals for phase, mesor, and amplitude as predicted by cosinor analysis under ketamine/xylazine anesthesia for male (blue) and female (red) animals. Midline: cosinor estimate, boxes: upper and lower 95% confidence interval boundaries. (c) Mean average intensity per animal over time under pentobarbital anesthesia. Individual animals are colored dots, solid lines indicate significant cosinor fit, dotted lines indicate insignificant fit. For cd: n = 27 female (12 day, 15 night) and 29 male (14 day, 15 night) mice; 4–6 animals per time point; (d) Min/max boxplot of average intensity during the day and night between male and female animals under pentobarbital anesthesia. Minima: minimum value, maxima: maximum value, center: median, quartiles: box and whisters. *p < 0.05. Individual mice represented by colored dots. (e) Mean average intensity per animal over time under avertin anesthesia. Individual animals are colored dots, solid lines indicate significant cosinor fit, dotted lines indicate insignificant fit. n = 25 female (13 day, 12 night) and 28 male (14 day, 14 night) mice, 2–6 per time point. (f) Min/max boxplot of average intensity during the day and night between male and female animals under avertin anesthesia. Minima: minimum value, maxima: maximum value, center: median, quartiles: box and whisters. *p < 0.05. Individual mice represented by colored dots.
