Figure 4
GSNO and MPP+ increased the levels of S-nitrosylated parkin and DMT1 in parkin-transfected SH-SY5Y cells. (a) Changes of SNO-parkin and DMT1 protein levels following different treatment (200 μM of MPP+; 200 μM of GSNO; 200 μM of MPP+ + 1 mM of L-NNA) for 24 h in parkin-transfected SH-SY5Y cells. (b) Statistical analysis for changes in levels of SNO-parkin. Data were presented as the ratio of SNO-parkin/parkin. ***P = 0.0002, parkin-GSNO versus parkin-GSH; ^^^P = 0.0002, parkin-MPP+ versus parkin-NS; ###P = 0.0004, parkin-MPP+ + L-NNA versus parkin-MPP+; P = 0.0541, parkin-MPP+ versus parkin-GSNO. (c) Statistical analysis for changes in levels DMT1. Data were presented as the ratio of DMT1/β-actin. GSNO treatment acted as a positive control for MPP+ treatment. ***P = 0.0004, parkin-GSNO versus parkin-GSH; ^^P = 0.0027, parkin-MPP+ versus parkin-NS; ##P = 0.0081, parkin-MPP+ + L-NNA versus parkin-MPP+; P = 0.847, parkin-MPP+ versus parkin-GSNO. N = 9.
