Figure 2

Polyacrylamide gel electrophoresis (PAGE) with silver staining of fossil and modern GuHCl extracts. (A) Fossil bone (Edmontosaurus fibula SRHS-DU-231) and sediment GuHCl extracts were loaded at 4 mg of extraction yield per lane and extract resuspension buffers were run as a negative control. Orange staining is seen as a smear at high molecular weights in the fossil bone GuHCl lane (arrow). Dark brown bands in the buffer lane adjacent to the molecular weight markers were caused by slight carryover of the weight markers solution during loading. (B) Silver-stain results for modern control Alligator HCl and GuHCl extracts loaded at 20 µg/lane.