Figure 6
The enriched “FcγR dependent phagocytosis” gene pathway and antibody-dependent phagocytosis assays reveal antibody-dependent cellular phagocytosis (ADCP) and antibody-dependent neutrophil phagocytosis (ADNP) in the lesions of CL patients. Gene networks related to “FcγR dependent phagocytosis” pathway was enriched in the GSEA in skin lesions from UCL (a) and NUCL (b) patients. The dot colors indicate fold change in the gene expression as stated in the map. Violin plots show L. tropica-specific IgG antibody levels (ng/mL) in skin biopsy supernatants (c) and serum samples (d), ADCP activity in skin biopsy supernatants (e) and serum samples (f), as well as ADNP activity in skin biopsy supernatants (g) and serum samples (h). The phagocytic activity (AUC) was measured by the uptake of L. tropica-specific antibody-bead complexes by either human peripheral neutrophils or THP-1 cells, using flow cytometry. Spearman correlation between IgG antibody levels and ADCP, in skin biopsies (i) and serum samples (j), as well as between IgG antibody levels and ADNP, in skin biopsies (k) and serum samples (l). All figures were created using R version 3.5.1.
