Figure 4

ARA-, DHA- and EPA-derived metabolites in the heart treated with Omega-3 EE. Arachidonic acid (ARA) (a), docosahexaenoic acid (DHA) (b), and eicosapentaenoic acid (EPA) (c) -related metabolites in heart were examined before (0), and 1 and 2 weeks (wks) after TAC surgery using liquid chromatography-tandem mass spectrometry. n = 3–6. *p < 0.05 compared between vehicle and Omega-3 EE groups. #p < 0.05 compared with vehicle group before TAC, †p < 0.05 compared with Omega-3 EE group before TAC. PG, Prostaglandin. HETE, Hydroxyeicosatetraenoic acid. TX, Thromboxane. HDoHE, Hydroxydocosahexaenoic acid. PD, Protectin D. Rv, Resolvin. HEPE, Hydroxyeicosapentaenoic acid. (d) MDA concentration in the heart was evaluated using TBARS assay. n = 4–7.