Figure 10
From: Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells
Effect of EVs supplementation on the level of granulosa cells apoptosis. Supplementation of HS-EVs showed to have deleterious effect granulosa cells by decreasing the proportion of viable cells and increasing the proportion of early apoptotic cells when the cells were cultured at 37 °C (A). However, supplementation of HS-EVs showed to have a beneficial effect by increasing the proportion of viable cells and decrease the proportion of early and late apoptotic cells when the cells were subsequently exposed to HS (B). The upper panels show the distribution of cells stained for both Annexin V and PI across the different quadrants. Data are presented as mean ± SEM and the mean differences were analyzed using one-way ANOVA followed by Bonferroni’s Multiple Comparison Test. **p < 0.01; ***p < 0.001, ns not significant.
