Figure 2

Impact of HS on the expression of HSPs and protein oxidation. The transcript and protein abundance of the HSPs using qRT-PCR and western blot, respectively. Elevated expression of the HSP90 (A), HSP70 (B) was observed in HS cells. The summary of the western blot analysis showed significant upregulation of the HSP90 (C) and HSP70 (D). The band strength of the HSP90 and HSP70 in each replicate is also indicated (E). The impact of HS on the amount of total oxidized protein was examined and cells exposed to HS had a significantly higher amount of oxidized proteins (F). Data are presented as mean ± SEM and the mean differences were analyzed using the Two-tail student’s t test. *p < 0.05, **p < 0.01; ***p < 0.001. Full-length blots are presented in Supplementary Figure S5.