Figure 1

CD151 expression is upregulated on T cells following TCR activation. CD151 expression on CD4+ and CD8+ T cells in PBMCs was determined at baseline or following activation with an anti-CD3/CD28 mAb combination (OKT3/CD28.2) using flow cytometric analysis. Representative flow plots for (a) CD4+ and (c) CD8+ T cells showing the increase in the CD151 expression frequency following stimulation. The percentage of CD151+ (b) CD4+ and (d) CD8+ T cells in the peripheral blood of 11 healthy individuals at baseline (gray circles) and on day 4 following stimulation with an anti-CD3/CD28 mAb combination (black circles) presented as a beeswarm plot. The red lines represent the median values. PBMC from 2 healthy volunteers were stimulated with anti-CD3/CD28 mAb (black line) or with SEB (blue line), and CD151 expression was determined on (e) CD4+ T cells and (f) CD8+ T cells over a period of 72 h. CD151 expression on unstimulated cells is depicted by gray circles. (g) Twenty-four hours after anti-CD3/CD28 mAb stimulation, the increase in relative CD151 mRNA expression in CD4+ T cells was measured using qRT-PCR. (h) Representative histograms of PBMC stimulated with anti-CD3/CD28 mAb and treated with brefeldin A (purple line) or monensin (gray line) after 6 h to block cell surface transport of newly synthesized proteins. CD151 expression was then determined 24 h post activation. The black histograms represent stimulated cultures with no transport inhibitor treatment. (i) CD4+CD151 + and CD8+CD151+ T cell frequencies in PBMCs from six donors 24 h post anti-CD3/CD28 stimulation cultured in either the absence or presence of brefeldin A or monensin treatment.