Figure 2

CD151 expression as a function of T cell proliferation. (a) CFSE-labeled PBMCs were stimulated with either a CMV pp65 antigen peptide library or anti-CD3/CD28 mAbs. Four days post stimulation, cell proliferation was determined as the reduction of the CFSE signal in relation to the regulation of CD151 expression using flow cytometric analysis. Flow plots show CD151 expression on proliferating CD4+ T cells (CFSE^lo) and non-proliferating CD4+ T cells. The depicted experiment is representative for four tested donors. PBMC from four healthy individuals were stimulated with anti-CD3/CD28 mAb (black lines) or left unstimulated (media, gray lines) for various lengths of time and the expression of CD151 on proliferating cells (CFSE^lo) was determined for (b) CD4+ T cells and (c) CD8+ T cells. Unstimulated cells served the negative controls.