Figure 3

Mutation types of waxy gene. (a) Wild-type waxy gene is about 4.5 kb long and contains 14 exons numbered e1 to e14. Important gene cassettes in promoter region as well as the start and stop codons are indicated. Arrows above schematic of waxy gene mark sites of insertion mutations; lines below mark sites of deletion mutations. wx-hAT is a new mutation identified in this study. (b) Detection of wx-hAT mutation by 1% agarose electrophoresis of PCR products. M, marker; lane 1–9, homozygous-type wx-hAT; lane 10–12, wild-type waxy gene. (c) Structural features of wx-hAT waxy gene mutation. wx-hAT is 2286 bp long, and contains a partial-length of the hAT element (grey box), 8-bp TSD (red box), 3-bp (yellow box) and 9-bp (green box) TIRs.