Figure 2

Orthogonal partial least squares discriminant analysis (PLS-DA) models for the classification into resistant/partial resistant and susceptible groups using of untargeted metabolomics data obtained in positive (ESI+) and negative (ESI−) ion modes. (a,b) Score plots for the predictive and first orthogonal components. Squares represent wild Vitis, while circles represent domesticated V. vinifera. Confidence ellipses are drawn for the two classification groups: resistant/partial resistant (blue) and susceptible (red); (c,d) Significance diagnostic showing the distribution of predictive accuracy in permutation tests and the p-value of the test for accuracy. 1000 permutations were randomly sampled. Vertical lines indicate the accuracy of model with labels non-permuted. Accuracy was estimated by sevenfold stratified cross-validation. Vitis genotypes abbreviations are indicated in Table 1.