Table 2 Candidate reference genes for qPCR.
Gene (NCBI accession number) | Primer sequence | Amplicon length (bp) | Ta (°C) | Tm (°C) |
|---|---|---|---|---|
60S (XM_002270599.3) | Fw: ATCTACCTCAAGCTCCTAGTC Rev: CAATCTTGTCCTCCTTTCCT | 165 | 60 | 79.6 |
TTC7B (XM_002283371.4) | Fw: GCTCTGTTGTTGAAGATGGG Rev: GGAAGCAGTTTGTAGCATCAG | 156 | 60 | 79.9 |
EF1α (XM_002284888.3) | Fw: GAACTGGGTGCTTGATAGGC Rev: ACCAAAATATCCGGAGTAAAAGA | 164 | 60 | 79.7 |
UBQ (XR_002030722.1) | Fw: GCCCTGCACTTACCATCTTTAAG Rev: GAGGGTCGTCAGGATTTGGA | 75 | 60 | 78.9 |
SAND (XM_002285134.3) | Fw: CAACATCCTTTACCCATTGACAGA Rev: GCATTTGATCCACTTGCAGATAAG | 76 | 60 | 79.2 |
GAPDH (XM_002263109.3) | Fw: TCAAGGTCAAGGACTCTAACACC Rev: CCAACAACGAACATAGGAGCA | 226 | 60 | 81.3 |
ACTINa (XM_019223591.1) | Fw: ATTCCTCACCATCATCAGCA Rev: GACCCCCTCCTACTAAAACT | 89 | 55 | 77.5 |
α-TUB (XM_002285685.4) | Fw: CAGCCAGATCTTCACGAGCTT Rev: GTTCTCGCGCATTGACCATA | 119 | 60 | 78.8 |
AP2M (XM_002281261.3) | Fw: CCTCTCTGGAATGCCTGATTT Rev: CTTTAGCAGGACGGGATTTA | 89 | 55 | 75.0 |
β-TUB (XM_002275270.3) | Fw: TGAACCACTTGATCTCTGCGACTA Rev: CAGCTTGCGGAGGTCTGAGT | 86 | 60 | 82.3 |
