Figure 1
From: A highly sensitive and specific real-time quantitative PCR for BRAF V600E/K mutation screening
Design and performance of the improved BRAF V600E/K mutation-specific RT-qPCR. (A) Sequences and relative locations of the primers and probe used in the assay. Amplification plots and standard curves for mutant alleles of BRAF V600E (B) and V600K (C). The standard curve of threshold cycle values were plotted against the log of copy numbers of mutant alleles and labeled with standard error bars. Each data point represents an average of three detection replicates for each dilution and the linearity is valid over four logs. The amplification plots were snapshots from the Qiagen Rotor-gene Q series software. Other parts of the figure were created using Microsoft Office 2013.
