Figure 2
From: A highly sensitive and specific real-time quantitative PCR for BRAF V600E/K mutation screening
BRAF V600E/K mutation-specific RT-qPCR, Sanger sequencing, V600E specific immunohistochemistry and histology staining results for representative cases of lung cancer (A) and thyroid cancer (B). Figures in the first column are qPCR amplification plots. Green, orange and yellow curves represent signals corresponding to BRAF V600E/K alleles, BRAF wild-type allele and CYP17 genes, respectively. Chromatograms in the second column are Sanger sequencing results and mutation sites are marked with red arrows. Images in the third and fourth columns are immunohistochemistry staining and hematoxylin/eosin staining results, respectively. The amplification plots and chromatograms of Sanger sequencing were snapshots from the Qiagen Rotor-gene Q series software and Chromas lite (v2.21, https://www.technelysium.com.au/), respectively. The immunohistochemistry and hematoxylin/eosin results were captured using Olympus BX51TF microscope and DP80 camera with cellSens software under × 40 and × 10 magnification, respectively.
