Figure 6
Pax6 controls the proper attachment and neurite outgrowth in the retinal explants. (A) Schematic of the methodology adopted to perform this experiment. (B–F) Co-culturing retinal explants from control GFP and Pax6−/− embryos (B–D) Pax6−/− retinas displayed poor attachment to the culture dish (arrowheads B and D). (D) Pax6−/− retinal tissue was forced down with coverslip but still lacked attachment potential. (E and F) GFP and Pax6fl/fl embryos co-cultured together showed robust axon and neurite outgrowth displaying proper attachment (Arrows, B & C, E & F). Scale bar—100 µm. (G and H) β-III tubulin stained retinal explants from Pax6fl/fl and Pax6−/− retina, showed altered axonal outgrowth and fasciculation in Pax6−/− retinas. n = 3, 3 independent explant culture preparations were made. Scale bar—100 µm.
