Figure 1
Experiment design and [18F]F-FDG PET-CT imaging protocol: Three pre-weighed fasted wild-type (WT) C57BL/6J mice were injected intravenously (i.v.) with 2–5 MBq of [18F]F-FDG. Each mouse was then prepared for isoflurane anaesthesia and imaged. After baseline imaging, mice were exposed to 0.05 ppm of ozone (O3) for 2 h. After exposure i.e. O3-0 h, the mouse was prepared for follow-up imaging of residual [18F]F-FDG activity. The mice were brought back to their cages and left in room-air. At 24 h after O3 exposure, the mice were injected with another intravenous dose of 2–5 MBq of [18F]F-FDG and imaged (O3-24 h). After imaging, mice were returned to their cages. After 4 h, i.e. O3-28 h, the mice were imaged again for residual [18F]F-FDG activity.
